Scaling with ranked subsampling (SRS) curve of phyloseq object

Usage

SRS_curve_pq(physeq, clean_pq = FALSE, ...)

Arguments

physeq

(required): a phyloseq-class object obtained using the phyloseq package.

clean_pq

(logical): Does the phyloseq object is cleaned using the clean_pq() function?

...

Other arguments passed on to SRS::SRScurve()

Value

A plot

Examples

if (requireNamespace("SRS")) {
  SRS_curve_pq(data_fungi_mini,
    max.sample.size = 200,
    rarefy.comparison = TRUE, rarefy.repeats = 3
  )
  SRS_curve_pq(data_fungi_mini, max.sample.size = 500, metric = "shannon")
}
#> Loading required namespace: SRS
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 829
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 50
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 36
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 46
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 157
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 39
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 43
#> Warning: function should be used for observed counts, but smallest count is 43
#> Warning: function should be used for observed counts, but smallest count is 43
#> Warning: function should be used for observed counts, but smallest count is 43
#> Warning: function should be used for observed counts, but smallest count is 43
#> Warning: function should be used for observed counts, but smallest count is 43
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 13
#> Warning: function should be used for observed counts, but smallest count is 13
#> Warning: function should be used for observed counts, but smallest count is 13
#> Warning: function should be used for observed counts, but smallest count is 13
#> Warning: function should be used for observed counts, but smallest count is 13
#> Warning: function should be used for observed counts, but smallest count is 13
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 520
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 4
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 182
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 9
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 21
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 18
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 3
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 7
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 471
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 5
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 68
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 6
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 2
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129
#> Warning: function should be used for observed counts, but smallest count is 129